Retrograde Ejaculation

In some men, the semen passes back into the bladder at ejaculation - this is confirmed by examination of a sample of post-ejaculatory urine.
The man must take sodium bicarbonate the day before, and the day of, his appointment – to alkalinize his urine.
Before collection, he should pass urine and then wait until he feels there is some urine in his bladder before masturbating.
He then collects a urine sample for analysis.
Assess volume and pH of the urine.
Centrifuge (600g for 10 min), resuspend pellets to a total of 20ml with sperm buffer + protein.
Re-spin, resuspend pellets and combine – add buffer to a final volume of 1ml.
Perform a standard semen analysis with this suspension.