Sperm Morphology Examination


Morphology describes the shape and size of sperm. At least 30% of sperm should be oval shaped, and usually up to 70% of sperm are abnormally shaped in some way.

  • Morphology is even more important than motility and concentration.
  • Because of the small size of the human sperm head, must use an air-dried smear which has been stained.
  • Prepared samples are assessed using a 100× oil-immersion objective under bright field optics.
  • The WHO recommends that 200 spermatozoa are counted per sample (and says that 2 × 200 is better).
  • Fields for counting must be selected at random.
  • When counting, remember about the normal distribution.